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The relaxant effect of nicardipine on the isolated uterine smooth muscle of the pregnant rat
Anesth Pain Med 2019;14(4):429-33
Published online October 31, 2019
© 2019 Korean Society of Neuroscience in Anesthesiology and Critical Care.

Dong Joon Kim1,2 , Mi Ha Hwang1 , Tae Hun An1,2 , and Ki Tae Jung1,2
Department of Anesthesiology and Pain Medicine, 1Chosun University Hospital, 2Chosun University School of Medicine, Gwangju, Korea
Correspondence to: Ki Tae Jung, M.D., Ph.D. Department of Anesthesiology and Pain Medicine, Chosun University Hospital, Chosun University School of Medicine, 365 Pilmun-daero, Dong-gu, Gwangju 61453, Korea Tel: 82-62-220-3223 Fax: 82-62-223-2333 E-mail: mdmole@chosun.ac.kr
ORCID https://orcid.org/0000-0002-2486-9961
Received April 24, 2019; Accepted June 4, 2019.
cc This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Nicardipine, a calcium channel blocker, is used to treat hypertension in pregnancy or preterm labor. The current study was conducted to investigate the relaxant effects of nicardipine on the isolated uterine smooth muscle of the pregnant rat.
Methods: We obtained uterine smooth muscle strips from pregnant female SD rats. After uterine contraction with oxytocin 10 mU/ml, we added nicardipine (10−12 to 10−8 M) accumulatively every 20 min. We recorded active tension and frequency of contraction, and calculated EC5 (effective concentration of 5% reduction), EC25, EC50, EC75, and EC95 of active tension and frequency of contraction using a probit model.
Results: Nicardipine (10−12 to 10−8 M) decreased active tension and frequency of contraction in a concentration-dependent manner. The EC50 and EC95 of nicardipine in the inhibition of active tension of the uterine smooth muscle were 2.41 × 10−10 M and 3.06 × 10−7 M, respectively. The EC50 and EC95 of nicardipine in the inhibition of frequency of contraction of the uterine smooth muscle were 9.04 × 10−11 and 4.18 × 10−7 M, respectively.
Conclusions: Nicardipine relaxed and decreased the frequency of contraction of the uterine smooth muscle in a concentration-dependent pattern. It might be possible to adjust the clinical dosage of nicardipine in the obstetric field based on our results, but further clinical studies are needed to confirm them.
Key Words : Nicardipine; Rat; Relaxation; Smooth muscle; Uterine contraction; Uterus.


October 2019, 14 (4)
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  • Korean Society of Obstetric Anesthesiologists